Reference tool · For research use only

Peptide Reconstitution & Dosage Calculator

Turn a lyophilized vial into an exact, verifiable draw on a U-100 insulin syringe. Built by a manufacturer — because the math only holds when the vial truly contains what the label says.

99%+ purity Third-party tested · Janoshik COA · HPLC · LC-MS/MS per batch

Calculator

Configure your protocol

Enter the numbers from your vial. Everything updates live — no guessing, no submit button.

GLP-1 toolkit

Titration schedule reference

The most-asked GLP-1 question — how to ramp up week by week. Pick a compound to see a commonly cited schedule, then send any dose straight to the calculator above.

Schedules are compiled from published clinical-consensus guidelines for research reference only — not a treatment plan. Always confirm against current prescribing information.

Phase Dose Frequency

GLP-1 toolkit

Choosing between the GLP-1 compounds

Mechanism, reported trial weight-loss, and dosing rhythm side by side — so buyers can tell the molecules apart.

Weight-loss figures are readouts from the named published trials, shown for reference only — not outcomes to expect from any individual protocol.

Compound Mechanism Reported peak loss Frequency Noted strength
Semaglutide GLP-1 agonist −14.9%
STEP 1, 68 wk (2.4 mg)
Weekly Longest real-world safety track record
Tirzepatide GIP / GLP-1 dual −22.9%
SURMOUNT-1 (15 mg)
Weekly Highest reported weight reduction of the class
Mazdutide GCG / GLP-1 dual −14.8%
GLORY-1, 48 wk (6 mg)
Weekly Reported liver-fat reduction ~80% in trial
Retatrutide GCG / GIP / GLP-1 triple Investigational
Phase studies ongoing
Weekly Studied for weight and hepatic-fat endpoints

How it works

Understanding the math

Three ideas remove almost every dosing mistake.

mg and mcg — get the decimal right

1 mg = 1,000 mcg. This one conversion is behind most beginner errors. A “0.25 mg” protocol is 250 mcg; “500 mcg” is 0.5 mg. When unsure, work entirely in mcg.

What a “Unit” means

On a U-100 insulin syringe, 100 Units = 1 mL. A Unit measures liquid volume, not peptide mass — it tells you how much fluid to draw, never how many milligrams. Concentration is what links the two.

The worked example

A 10 mg vial with 2 mL water: 10,000 mcg ÷ 200 Units = 50 mcg per Unit. For a 250 mcg dose: 250 ÷ 50 = 5 Units. Add more water and each Unit carries less — handy for very small doses.

Handling

Storage & reconstitution — the science, cited

Freeze-drying slows degradation by orders of magnitude, but never stops it. Moisture matters as much as temperature.

Lyophilized powder (unmixed)

Keep sealed, dry, and out of direct light. Our product guidance: 2–8°C for up to 24 months; −20°C for up to 36 months for maximum stability — always defer to the batch COA. Freeze-drying locks the peptide into an amorphous glassy state where it barely moves; controlling residual moisture is as important as temperature.

Lai & Topp, J Pharm Sci 1999 · Duddu & Dal Monte, Pharm Res 1997

After reconstitution (liquid)

Once bacteriostatic water is added, refrigerate at 2–8°C. Reconstituted peptides are stable on the order of weeks, not months — plan use accordingly and follow the COA. Do not re-freeze a reconstituted vial; repeated freeze–thaw accumulates aggregates.

Bauer et al., Pharm Res 2018

The reconstitution technique

Peptide chains are fragile. Aim the water stream at the glass wall so it runs down gently onto the cake — never blast it directly into the powder. Don’t shake; swirl or roll the vial between your fingers until fully dissolved.

Supplies

  • Bacteriostatic water (0.9% benzyl alcohol) for multi-use vials; sterile water is single-use only.
  • U-100 insulin syringes, typically 31G × 8 mm (5/16") for subcutaneous draws.
  • Alcohol prep pads for the stopper and site.

Reference

Research reference library

Categories, reported research dose ranges, and studied directions — compiled from published literature for orientation only. Not dosing guidance.

Dose ranges are compiled from published research literature for orientation only — not dosing guidance.

Compound Category Reported research range Route Studied directions
Semaglutide GLP-1 & Metabolic 0.25 – 2.4 mg / week Sub-Q Studied for glycemic regulation and body-weight reduction
Tirzepatide GLP-1 & Metabolic 2.5 – 15 mg / week Sub-Q Studied for dual GIP/GLP-1 metabolic and weight endpoints
Retatrutide GLP-1 & Metabolic 1 – 12 mg / week Sub-Q Investigational triple agonist; studied for weight and hepatic-fat endpoints
BPC-157 Repair & Recovery 250 – 500 mcg / day Sub-Q Studied in models of tendon, ligament and gut-tissue repair
TB-500 (TB-4 fragment) Repair & Recovery 2 – 5 mg / week Sub-Q Studied for tissue repair and cell migration
NAD+ Repair & Recovery 50 – 100 mg / day Sub-Q / IV Studied in cellular-energy and NAD-metabolism research
Ipamorelin GH Secretagogues 200 – 300 mcg / dose Sub-Q Studied as a selective GH secretagogue
CJC-1295 (no DAC) GH Secretagogues 100 – 200 mcg / dose Sub-Q Studied for GHRH-analog GH release, often paired with a GHRP
Tesamorelin GH Secretagogues 1 – 2 mg / day Sub-Q Studied for GHRH-analog activity and visceral-adipose endpoints
MOTS-c Other Research Peptides 5 – 10 mg / week Sub-Q Mitochondrial-derived peptide; studied in metabolic-regulation research
PT-141 (Bremelanotide) Other Research Peptides 0.5 – 2 mg / dose Sub-Q Melanocortin agonist; studied for sexual-function endpoints

FAQ

Common questions

Bacteriostatic water vs. sterile water — does it matter?

Yes. Bacteriostatic water contains 0.9% benzyl alcohol, which lets a multi-use vial be drawn from over several weeks under refrigeration. Sterile water has no preservative — a vial reconstituted with it should be treated as single-use.

If I add more water, do I get less peptide?

No. The total peptide mass in the vial is fixed. Adding more water only lowers the concentration, so you draw more Units for the same dose. Extra water can actually make very small doses easier to measure accurately.

Why does purity matter for this calculator?

Every figure here assumes the vial holds exactly the labeled mass. If a vial is underfilled or impure, the real dose drifts from the calculated one. That is why each of our batches ships with a Certificate of Analysis and third-party (Janoshik) HPLC / LC-MS/MS results.

What syringe should I use?

A U-100 insulin syringe, on which 100 Units equals 1 mL. A Unit is a mark of liquid volume, not peptide mass. For subcutaneous draws a 31G × 8 mm (5/16") needle is common and comfortable.

How long is a reconstituted vial good for?

Refrigerate reconstituted peptides at 2–8°C and plan use on the order of weeks, not months. Do not re-freeze a reconstituted vial — repeated freeze–thaw accumulates aggregates. Always defer to the batch Certificate of Analysis.

Medical & legal disclaimer

For research and reference use only. This calculator performs unit-conversion arithmetic and does not constitute medical advice, a recommendation to administer any substance, or a claim of safety or efficacy for any use in humans or animals. Our products are supplied as research materials. Reference ranges shown are compiled from published research literature for orientation only and are not dosing instructions. Verify every calculation independently and consult a qualified professional before any use. We disclaim liability for reliance on this tool.